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SRGAP2 Antibody

CAB19512
Assay Genie
TargetUBE2D1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    SRGAP2 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID7321
  • Target name
    UBE2D1
  • Target description
    ubiquitin conjugating enzyme E2 D1
  • Target synonyms
    E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A, ubiquitin-conjugating enzyme E2 D1, (E3-independent) E2 ubiquitin-conjugating enzyme D1, E2 ubiquitin-conjugating enzyme D1, UBC4/5 homolog, stimulator of Fe transport, ubiquitin carrier protein D1, ubiquitin conjugating enzyme E2D 1, ubiquitin-conjugating enzyme E2(17)KB 1, ubiquitin-conjugating enzyme E2-17 kDa 1, ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast), ubiquitin-protein ligase D1
  • Host
    Rabbit
  • Protein IDP51668
  • Protein Name
    Ubiquitin-conjugating enzyme E2 D1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: UBE2D1 antibody at 2microg/ml Lane 1: Mouse thymus tissue Lane 2: NIH/3T3 whole cell lysate Lane 3: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 17 kDa Observed band size: 17 kDa
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Figure 1. Western blot analysis of UBE2D1/2/3/4 using anti-UBE2D1/2/3/4 antibody (A04728-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2D1/2/3/4 antigen affinity purified polyclonal antibody (Catalog # A04728-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBE2D1/2/3/4 at approximately 17KD. The expected band size for UBE2D1/2/3/4 is at 17KD.
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