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Western blot All lanes: SRI antibody at 2microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit at 1/10000 dilution Predicted band size: 22, 21 kDa Observed band size: 22 kDa
Western blot All lanes: SRI antibody at 2microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit at 1/10000 dilution Predicted band size: 22, 21 kDa Observed band size: 22 kDa
Western blot All lanes: SRI antibody at 2microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit at 1/10000 dilution Predicted band size: 22, 21 kDa Observed band size: 22 kDa

SRI Antibody

CSB-PA022665ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSRI
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Overview

  • Supplier
    Cusabio
  • Product Name
    SRI Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6717
  • Target name
    SRI
  • Target description
    sorcin
  • Target synonyms
    CP-22, CP22, SCN, V19, sorcin, 22 kDa protein, H_RG167B05.1, calcium binding protein amplified in mutlidrug-resistant cells
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30626
  • Protein Name
    Sorcin
  • Scientific Description
    Calcium-binding protein that modulates excitation-contraction coupling in the heart. Contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Modulates the activity of RYR2 calcium channels.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SRI using anti-SRI antibody (A00222-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRI antigen affinity purified polyclonal antibody (Catalog # A00222-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRI at approximately 22 kDa. The expected band size for SRI is at 22 kDa.
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