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SRP72 Antibody

LS-C497607
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSRP72
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SRP72 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 67kDa/74kDa, while the observed MW by Western blot was 75kDa.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    Flo (1:20 - 1:50), IF (1:20 - 1:50), IHC (1:20 - 1:50), WB (1:500 - 1:2000) The predicted MW is 67kDa/74kDa, while the observed MW by Western blot was 75kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6731
  • Target name
    SRP72
  • Target description
    signal recognition particle 72
  • Target synonyms
    BMFF, BMFS1, HEL103, signal recognition particle subunit SRP72, epididymis luminal protein 103, signal recognition particle 72 kDa protein, signal recognition particle 72kD, signal recognition particle 72kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SRP72 using anti-SRP72 antibody (A05873-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: mouse pamcreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRP72 antigen affinity purified polyclonal antibody (Catalog # A05873-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRP72 at approximately 75 kDa. The expected band size for SRP72 is at 75 kDa.
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