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ProductsBack/SRPR antibody [C1C3]
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using SRPR(GTX105721) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using SRPR(GTX105721) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using SRPR(GTX105721) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

SRPR antibody [C1C3]

GTX105721
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetSRPRA
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Overview

  • Supplier
    GeneTex
  • Product Name
    SRPR antibody [C1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.37 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6734
  • Target name
    SRPRA
  • Target description
    SRP receptor subunit alpha
  • Target synonyms
    DP, SRPR, Sralpha, signal recognition particle receptor subunit alpha, DP-alpha, SR-alpha, SRP receptor alpha subunit, SRP-alpha, docking protein alpha, signal recognition particle receptor (docking protein)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08240
  • Protein Name
    Signal recognition particle receptor subunit alpha
  • Scientific Description
    The gene encodes a subunit of the endoplasmic reticulum signal recognition particle receptor that, in conjunction with the signal recognition particle, is involved in the targeting and translocation of signal sequence tagged secretory and membrane proteins across the endoplasmic reticulum. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SRPRA using anti-SRPRA antibody (A12340-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRPRA antigen affinity purified polyclonal antibody (Catalog # A12340-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRPRA at approximately 70 kDa. The expected band size for SRPRA is at 70 kDa.
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