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ProductsBack/SRPR beta (SRPRB) Mouse Monoclonal Antibody [Clone ID: OTI1D9]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SRPRB (Cat# RC209486, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SRPRB(Cat# TA504712). Positive lysates [LY402853] (100ug) and [LC402853] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SRPRB (Cat# RC209486, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SRPRB(Cat# TA504712). Positive lysates [LY402853] (100ug) and [LC402853] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SRPRB (Cat# RC209486, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SRPRB(Cat# TA504712). Positive lysates [LY402853] (100ug) and [LC402853] (20ug) can be purchased separately from OriGene.

SRPR beta (SRPRB) Mouse Monoclonal Antibody [Clone ID: OTI1D9]

TA504712
OriGene
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSRPRB
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    SRPR beta (SRPRB) Mouse Monoclonal Antibody [Clone ID: OTI1D9]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1D9
  • Gene ID58477
  • Target name
    SRPRB
  • Target description
    SRP receptor subunit beta
  • Target synonyms
    APMCF1, SR-beta, signal recognition particle receptor subunit beta, SRP receptor beta subunit, signal recognition particle receptor, B subunit, signal recognition particle receptor, beta subunit
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9Y5M8
  • Protein Name
    Signal recognition particle receptor subunit beta
  • Scientific Description
    SRPRB mouse monoclonal antibody, clone OTI1D9 (formerly 1D9)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of SRPRB using anti-SRPRB antibody (A13302-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRPRB antigen affinity purified polyclonal antibody (Catalog # A13302-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRPRB at approximately 30 kDa. The expected band size for SRPRB is at 30 kDa.
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