HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with GTX89811 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with GTX89811 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with GTX89811 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with GTX89811 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
SSA1 antibody, C-term
GTX89811
Overview
- SupplierGeneTex
- Product NameSSA1 antibody, C-term
- Delivery Days Customer7
- Application Supplier NoteWB: 0.2-1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.50 mg/ml
- ConjugateUnconjugated
- Gene ID6737
- Target nameTRIM21
- Target descriptiontripartite motif containing 21
- Target synonyms52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro protein; E3 ubiquitin-protein ligase TRIM21; RING finger protein 81; RING-type E3 ubiquitin transferase TRIM21; RNF81; ro(SS-A); Ro/SSA; Ro/SSA 52kDa; RO52; Sicca syndrome antigen A; sjoegren syndrome type A antigen; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro); SSA; SS-A; SSA1; tripartite motif-containing protein 21
- HostGoat
- IsotypeIgG
- Protein IDP19474
- Protein NameE3 ubiquitin-protein ligase TRIM21
- Scientific DescriptionThis gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203