Bio-Connect

SSEA-5 antibody [8e11]

GTX70019
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    GeneTex
  • Product Name
    SSEA-5 antibody [8e11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    ICC/IF: 1:50-1:1000. FACS: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Mouse
  • Isotype
    IgG
  • Scientific Description
    Stage-specific embryonic antigen (SSEA)-5 is an H type-1 glycan that is highly and specifically expressed on human embryonic stem cells (hESCs), the human blastocyst inner cell mass and undifferentiated human pluripotent stem cells (hPSCs). Expression of SSEA-5 in hESCs and hPSCs rapidly decreases upon differentiation and is considered an indicator of undifferentiated PSCs. This SSEA-5 antibody is capable of identifying undifferentiated pluripotent stem cells in heterogeneous populations, and when used in conjunction with other pluripotency surface markers (CD9 and CD90 or CD50 and CD200), it can be used to immunodeplete residual teratoma-causing cells from incompletely differentiated embryonic stem cell cultures.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • A set of cancer stem cell homing peptides associating with the glycan moieties of glycosphingolipids. Su YH et al., 2018 Apr 17, Oncotarget
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  • Immunoreactivity of Pluripotent Markers SSEA-5 and L1CAM in Human Tumors, Teratomas, and Induced Pluripotent Stem Cells. Cassidy L et al., 2013, J Biomark
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  • An antibody against SSEA-5 glycan on human pluripotent stem cells enables removal of teratoma-forming cells. Tang C et al., 2011 Aug 14, Nat Biotechnol
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