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ProductsBack/SSR2 antibody [AT31G6]
WB analysis of Jurkat lysate (40ug) using SSR2 antibody at a dilution of 1:500.
WB analysis of Jurkat lysate (40ug) using SSR2 antibody at a dilution of 1:500.
WB analysis of Jurkat lysate (40ug) using SSR2 antibody at a dilution of 1:500.

SSR2 antibody [AT31G6]

GTX53784
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSSR2
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Overview

  • Supplier
    GeneTex
  • Product Name
    SSR2 antibody [AT31G6]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:500.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT31G6
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6746
  • Target name
    SSR2
  • Target description
    signal sequence receptor subunit 2
  • Target synonyms
    HSD25, TLAP, TRAP-BETA, TRAPB, translocon-associated protein subunit beta, SSR-beta, signal sequence receptor subunit beta, signal sequence receptor, beta (translocon-associated protein beta), translocon-associated protein beta
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP43308
  • Protein Name
    Translocon-associated protein subunit beta
  • Scientific Description
    The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein (alpha-SSR or SSR1) and a 22-kD glycoprotein (beta-SSR or SSR2). The human beta-signal sequence receptor gene (SSR2) maps to chromosome bands 1q21-q23. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of TRAPB/SSR2 using anti-TRAPB/SSR2 antibody (A11273-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human COLO 320 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAPB/SSR2 antigen affinity purified polyclonal antibody (Catalog # A11273-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAPB/SSR2 at approximately 22 kDa. The expected band size for TRAPB/SSR2 is at 20 kDa.
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