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SSR2 Antibody, HRP conjugated

CSB-PA022718LB01HU
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetSSR2
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Overview

  • Supplier
    Cusabio
  • Product Name
    SSR2 Antibody, HRP conjugated
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    HRP
  • Gene ID6746
  • Target name
    SSR2
  • Target description
    signal sequence receptor subunit 2
  • Target synonyms
    HSD25, TLAP, TRAP-BETA, TRAPB, translocon-associated protein subunit beta, SSR-beta, signal sequence receptor subunit beta, signal sequence receptor, beta (translocon-associated protein beta), translocon-associated protein beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43308
  • Protein Name
    Translocon-associated protein subunit beta
  • Scientific Description
    TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRAPB/SSR2 using anti-TRAPB/SSR2 antibody (A11273-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human COLO 320 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAPB/SSR2 antigen affinity purified polyclonal antibody (Catalog # A11273-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAPB/SSR2 at approximately 22 kDa. The expected band size for TRAPB/SSR2 is at 20 kDa.
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