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ProductsBack/SSRP1 Antibody - middle region (ARP31141_P050)

SSRP1 Antibody - middle region (ARP31141_P050)

ARP31141_P050
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
TargetSSRP1
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    SSRP1 Antibody - middle region (ARP31141_P050)
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  • Gene ID6749
  • Target name
    SSRP1
  • Target description
    structure specific recognition protein 1
  • Target synonyms
    FACT, FACT80, T160, FACT complex subunit SSRP1, FACT 80 kDa subunit, FACTp80, chromatin-specific transcription elongation factor 80 kDa subunit, cisplatin-DNA SSRP, facilitates chromatin remodeling 80 kDa subunit, facilitates chromatin transcription complex 80 kDa subunit, facilitates chromatin transcription complex subunit SSRP1, hSSRP1, high mobility group box, recombination signal sequence recognition protein 1
  • Host
    Rabbit
  • Reactivity
    Bovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SSRP1 using anti-SSRP1 antibody (A02606-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human DLD-1 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse testis tissue lysate, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSRP1 antigen affinity purified polyclonal antibody (Catalog # A02606-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSRP1 at approximately 92 kDa. The expected band size for SSRP1 is at 81 kDa.
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