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Western blot All lanes: ST6GALNAC2 antibody at 8ug/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa
Western blot All lanes: ST6GALNAC2 antibody at 8ug/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa
Western blot All lanes: ST6GALNAC2 antibody at 8ug/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa

ST6GALNAC2 Antibody

CSB-PA883452LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetST6GALNAC2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ST6GALNAC2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10610
  • Target name
    ST6GALNAC2
  • Target description
    ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 2
  • Target synonyms
    SAITL1, SIAT7, SIAT7B, SIATL1, ST6GalNAII, STHM, alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2, (alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide B, (alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide alpha-2,6-sialyltransferase, SIAT7-B, ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2, ST6 GalNAc alpha-2,6-sialyltransferase 2, ST6GALNAC2, alpha-2,6-sialyltransferase 2, ST6GalNAc II, ST6GalNAcII, galNAc alpha-2,6-sialyltransferase II, sialyltransferase 7 ((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide B, sialyltransferase 7 ((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide alpha-2,6-sialyltransferase) B, sialyltransferase 7B, sialyltransferase-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UJ37
  • Protein Name
    Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2
  • Scientific Description
    Golgi membrane, sialyltransferase activity, cellular protein metabolic process, dolichol-linked oligosaccharide biosynthetic process, ganglioside biosynthetic process, O-glycan processing, oligosaccharide metabolic process, post-translational protein modification, protein N-linked glycosylation via asparagine, protein O-linked glycosylation
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ST6GALNAC2 using anti-ST6GALNAC2 antibody (A10348). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse skeletal muscle tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST6GALNAC2 antigen affinity purified polyclonal antibody (Catalog # A10348) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST6GALNAC2 at approximately 40 kDa. The expected band size for ST6GALNAC2 is at 42 kDa.
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