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ProductsBack/ST7 Antibody - middle region (OAAB00506)

ST7 Antibody - middle region (OAAB00506)

OAAB00506
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Human, Mouse, Porcine, Rabbit, Rat, Zebra Fish
TargetST7
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    ST7 Antibody - middle region (OAAB00506)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Gene ID7982
  • Target name
    ST7
  • Target description
    suppression of tumorigenicity 7
  • Target synonyms
    ETS7q, FAM4A, FAM4A1, HELG, RAY1, SEN4, TSG7, suppressor of tumorigenicity 7 protein, family with sequence similarity 4, subfamily A, member 1, suppression of tumorigenicity 7 (breast)
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Bovine, Human, Mouse, Porcine, Rabbit, Rat, Zebra Fish
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ST7 using anti-ST7 antibody (A07235). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST7 antigen affinity purified polyclonal antibody (Catalog # A07235) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST7 at approximately 67 kDa. The expected band size for ST7 is at 67 kDa.
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