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WB analysis of various sample lysates using GTX64764 STAM antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64764 STAM antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64764 STAM antibody. Dilution : 1:1000 Loading : 25microg per lane

STAM antibody

GTX64764
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetSTAM
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Overview

  • Supplier
    GeneTex
  • Product Name
    STAM antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:200 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8027
  • Target name
    STAM
  • Target description
    signal transducing adaptor molecule
  • Target synonyms
    STAM-1, STAM1, signal transducing adapter molecule 1, HSE1 homolog, signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92783
  • Protein Name
    Signal transducing adapter molecule 1
  • Scientific Description
    This gene encodes a member of the signal-transducing adaptor molecule family. These proteins mediate downstream signaling of cytokine receptors and also play a role in ER to Golgi trafficking by interacting with the coat protein II complex. The encoded protein also associates with hepatocyte growth factor-regulated substrate to form the endosomal sorting complex required for transport-0 (ESCRT-0), which sorts ubiquitinated membrane proteins to the ESCRT-1 complex for lysosomal degradation. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Feb 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STAM1 using anti-STAM1 antibody (A00864-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAM1 antigen affinity purified polyclonal antibody (Catalog # A00864-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAM1 at approximately 59KD. The expected band size for STAM1 is at 59KD.
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