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Western Blot analysis of NIH-3T3 cells using STAM2 Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using STAM2 Polyclonal Antibody
Western Blot analysis of NIH-3T3 cells using STAM2 Polyclonal Antibody

STAM2 Antibody

CSB-PA004166
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSTAM2
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Overview

  • Supplier
    Cusabio
  • Product Name
    STAM2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10254
  • Target name
    STAM2
  • Target description
    signal transducing adaptor molecule 2
  • Target synonyms
    Hbp; Hrs-binding protein; HSE1 homolog; signal transducing adapter molecule 2; signal transducing adaptor molecule (SH3 domain and ITAM motif) 2; STAM-2; STAM2A; STAM2B; STAM-like protein containing SH3 and ITAM domains 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75886
  • Protein Name
    Signal transducing adapter molecule 2
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of STAM2 using anti-STAM2 antibody (A06533-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAM2 antigen affinity purified polyclonal antibody (Catalog # A06533-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAM2 at approximately 65-70 kDa. The expected band size for STAM2 is at 58 kDa.
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