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Western blot All lanes: STARD3 Antibody at 6ug/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51, 49, 50 kDa Observed band size: 51 kDa
Western blot All lanes: STARD3 Antibody at 6ug/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51, 49, 50 kDa Observed band size: 51 kDa
Western blot All lanes: STARD3 Antibody at 6ug/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51, 49, 50 kDa Observed band size: 51 kDa

STARD3 Antibody

CSB-PA618912DSR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSTARD3
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Overview

  • Supplier
    Cusabio
  • Product Name
    STARD3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10948
  • Target name
    STARD3
  • Target description
    StAR related lipid transfer domain containing 3
  • Target synonyms
    CAB1, MLN64, es64, stAR-related lipid transfer protein 3, MLN 64, START domain-containing protein 3, StAR-related lipid transfer (START) domain containing 3, metastatic lymph node gene 64 protein, metastatic lymph node protein 64, steroidogenic acute regulatory protein related
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14849
  • Protein Name
    StAR-related lipid transfer protein 3
  • Scientific Description
    Binds and transports cholesterol. Promotes steroidogenesis in placenta and brain.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MLN64/STARD3 using anti-MLN64/STARD3 antibody (A05385-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLN64/STARD3 antigen affinity purified polyclonal antibody (Catalog # A05385-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLN64/STARD3 at approximately 51 kDa. The expected band size for MLN64/STARD3 is at 51 kDa.
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Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
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