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STAT1 Antibody

ORB1242847
Biorbyt
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse
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Overview

  • Supplier
    Biorbyt
  • Product Name
    STAT1 Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    STAT1 antibody can be used for detection of STAT1 by ELISA at 1:312500. STAT1 antibody can be used for detection of STAT1 by western blot at 5.0 microg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    STAT1 antibody can be used for detection of STAT1 by ELISA at 1:312500. STAT1 antibody can be used for detection of STAT1 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Protein IDP42225
  • Protein Name
    Signal transducer and activator of transcription 1
  • Scientific Description
    STAT1 Antibody
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STAT1 using anti-STAT1 antibody (A00036-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT1 antigen affinity purified polyclonal antibody (Catalog # A00036-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT1 at approximately 91 kDa. The expected band size for STAT1 is at 87 kDa.
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