![WB analysis of HeLa cell extracts with/without TNF-alpha (20 ng/ml, 37oC, 30 min) treatment using GTX00959 STAT1 (phospho Ser701) antibody [GT1197]. Dilution : 1:1000 Loading : 25microg WB analysis of HeLa cell extracts with/without TNF-alpha (20 ng/ml, 37oC, 30 min) treatment using GTX00959 STAT1 (phospho Ser701) antibody [GT1197]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00959/GTX00959_20200327_WB_1_w_23053121_463.webp)
WB analysis of HeLa cell extracts with/without TNF-alpha (20 ng/ml, 37oC, 30 min) treatment using GTX00959 STAT1 (phospho Ser701) antibody [GT1197]. Dilution : 1:1000 Loading : 25microg
STAT1 (phospho Tyr701) antibody [GT1197]
GTX00959
ApplicationsWestern Blot
Product group Antibodies
TargetSTAT1
Overview
- SupplierGeneTex
- Product NameSTAT1 (phospho Ser701) antibody [GT1197] - Recombinant antibody, Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1197
- ConjugateUnconjugated
- Gene ID6772
- Target nameSTAT1
- Target descriptionsignal transducer and activator of transcription 1
- Target synonymsCANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91, signal transducer and activator of transcription 1-alpha/beta, signal transducer and activator of transcription 1, 91kD, signal transducer and activator of transcription 1, 91kDa, transcription factor ISGF-3 components p91/p84
- HostRabbit
- IsotypeIgG
- Protein IDP42224
- Protein NameSignal transducer and activator of transcription 1-alpha/beta
- Scientific DescriptionThe protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![WB analysis of PC-12 cell extracts with/without TNF-alpha (20 ng/ml, 37oC, 30 min) treatment using GTX00959 STAT1 (phospho Ser701) antibody [GT1197]. Dilution : 1:1000 Loading : 25microg WB analysis of PC-12 cell extracts with/without TNF-alpha (20 ng/ml, 37oC, 30 min) treatment using GTX00959 STAT1 (phospho Ser701) antibody [GT1197]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00959/GTX00959_20200327_WB_2_w_23053121_771.webp)
![WB analysis of NIH-3T3 cell extracts with/without TNF-alpha (20 ng/ml, 37oC, 30 min) treatment using GTX00959 STAT1 (phospho Ser701) antibody [GT1197]. Dilution : 1:1000 Loading : 25microg WB analysis of NIH-3T3 cell extracts with/without TNF-alpha (20 ng/ml, 37oC, 30 min) treatment using GTX00959 STAT1 (phospho Ser701) antibody [GT1197]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00959/GTX00959_20200327_WB_3_w_23053121_895.webp)
![Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT1 (phospho Ser701) antibody [GT1197] (GTX00959) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT1 (phospho Ser701) antibody [GT1197] (GTX00959) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00959/GTX00959_4000000049_20200313_WB_treatment_hIFN-alpha_w_23053121_963.webp)



![WB analysis of human malignant melanoma cells (short-term culture derived from a patient) treated with IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml) using GTX23987 STAT1 antibody [SM1]. Upper Lane : GTX23991 STAT1 (phospho Ser727) antibody [PSM1] Lower Lane : GTX23987 STAT1 antibody [SM1]](https://www.genetex.com/upload/website/prouct_img/normal/GTX23987/GTX23987_20191025_AP_001_184_w_23060722_761.webp)
![WB analysis of IFN-gamma treated HeLa cell lysate using GTX23991 STAT1 (phospho Ser727) antibody [PSM1]. Lane 1 : Immunoprecipitate by another anti-STAT1 antibody Lane 2 : Original whole cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX23991/GTX23991_20191025_AP_001_185_w_23060722_353.webp)

