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Western Blot analysis of various samples using STAT3 Monoclonal Antibody at dilution of 1:1500.
Western Blot analysis of various samples using STAT3 Monoclonal Antibody at dilution of 1:1500.
Western Blot analysis of various samples using STAT3 Monoclonal Antibody at dilution of 1:1500.

STAT3 Monoclonal Antibody

E-AB-70314
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    STAT3 Monoclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STAT3 using anti-STAT3 antibody (PB9511). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human SiHa whole cell lysates, Lane 7: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 antigen affinity purified polyclonal antibody (Catalog # PB9511) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for STAT3 at approximately 88 kDa. The expected band size for STAT3 is at 88 kDa.
Product group Antibodies
Boster Bio
Anti-STAT3 Antibody Picoband(r)PB9511-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetSTAT3
  • SizePrice
EB05073 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Product group Antibodies
Everest Biotech
Goat anti-STAT3 (isoform 1 and 2) AntibodyEB05073
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityBovine, Human, Mouse, Rat
TargetSTAT3
  • SizePrice
Product group Antibodies
RayBiotech
Anti-P-STAT3 Antibody130-10609
ApplicationsELISA
ReactivityHuman
TargetSTAT3
  • SizePrice
RSC96 cells probed with Rabbit Anti-STAT3 Polyclonal Antibody (bs-1141R),6ug, for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC)at 0.5ug(green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Product group Antibodies
References
Bioss
STAT3 Polyclonal AntibodyBS-1141R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetSTAT3
  • SizePrice
Product group Antibodies
References
GeneTex
STAT3 antibody [C3], C-termGTX104616
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Plant, Rat
TargetSTAT3
  • SizePrice
Product group Antibodies
Assay Genie
[KO Validated] STAT3 AntibodyCAB1192
ReactivityHuman
TargetSTAT3
  • SizePrice
Product group Antibodies
Biomatik
Stat3 Polyclonal AntibodyCAC08876
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
TargetSTAT3
  • SizePrice
Product group Antibodies
Biosynth
STAT3 antibody70R-20573
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Immunohistochemical staining of human prostate cancer shows strong nuclear and moderate cytoplasmic immunoreactivity in tumor cells.
Product group Antibodies
Atlas Antibodies
Anti-STAT3 AntibodyAMAB90776
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetSTAT3
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Anti-STAT3 Antibody
Product group Antibodies
Antibodies.com
Anti-STAT3 AntibodyA95775
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
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