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ProductsBack/STAT6 Recombinant Monoclonal Antibody
Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate All lanes: STAT6 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 95, 75, 82 kDa Observed band size: 100 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate All lanes: STAT6 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 95, 75, 82 kDa Observed band size: 100 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate All lanes: STAT6 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 95, 75, 82 kDa Observed band size: 100 kDa

STAT6 Recombinant Monoclonal Antibody

CSB-RA988543A0HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSTAT6
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Overview

  • Supplier
    Cusabio
  • Product Name
    STAT6 Recombinant Monoclonal Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2F10
  • Conjugate
    Unconjugated
  • Gene ID6778
  • Target name
    STAT6
  • Target description
    signal transducer and activator of transcription 6
  • Target synonyms
    D12S1644; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin4-induced; STAT6B; STAT6C; transcription factor IL-4 STAT
  • Isotype
    IgG
  • Protein IDP42226
  • Protein Name
    Signal transducer and activator of transcription 6
  • Scientific Description
    Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STAT6 using anti-STAT6 antibody (A00523-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT6 antigen affinity purified polyclonal antibody (Catalog # A00523-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT6 at approximately 110KD. The expected band size for STAT6 is at 110KD.
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