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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA618008LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA618008LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA618008LA01HU at dilution of 1:100

STIL Antibody

CSB-PA618008LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSTIL
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Overview

  • Supplier
    Cusabio
  • Product Name
    STIL Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6491
  • Target name
    STIL
  • Target description
    STIL centriolar assembly protein
  • Target synonyms
    MCPH7, SIL, SCL-interrupting locus protein, SCL/TAL1 interrupting locus, TAL-1-interrupting locus protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15468
  • Protein Name
    SCL-interrupting locus protein
  • Scientific Description
    Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Plays a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of STIL/SIL using anti-STIL/SIL antibody (A04956-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates, Lane 5: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STIL/SIL antigen affinity purified polyclonal antibody (Catalog # A04956-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STIL/SIL at approximately 170KD. The expected band size for STIL/SIL is at 170KD.
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Paraformaldehyde-fixed, paraffin embedded Rat testis tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with STIL Polyclonal Antibody, Unconjugated (bs-9303R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
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TargetSTIL
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