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Immunohistochemistry of paraffin-embedded human lymph node tissue using CSB-PA022829LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lymph node tissue using CSB-PA022829LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lymph node tissue using CSB-PA022829LA01HU at dilution of 1:100

STIM1 Antibody

CSB-PA022829LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSTIM1
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Overview

  • Supplier
    Cusabio
  • Product Name
    STIM1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6786
  • Target name
    STIM1
  • Target description
    stromal interaction molecule 1
  • Target synonyms
    D11S4896E; GOK; IMD10; STRMK; stromal interaction molecule 1; TAM; TAM1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13586
  • Protein Name
    Stromal interaction molecule 1
  • Scientific Description
    Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores (PubMed:15866891, PubMed:16005298, PubMed:16208375, PubMed:16537481, PubMed:16733527, PubMed:16766533, PubMed:16807233, PubMed:18854159, PubMed:19249086, PubMed:22464749, PubMed:24069340, PubMed:24351972, PubMed:24591628, PubMed:26322679, PubMed:25326555). Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit ORAI1 (PubMed:16208375, PubMed:16537481). Involved in enamel formation (PubMed:24621671). Activated following interaction with TMEM110/STIMATE, leading to promote STIM1 conformational switch (PubMed:26322679).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of STIM1 using anti-STIM1 antibody (A00312-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STIM1 antigen affinity purified polyclonal antibody (Catalog # A00312-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STIM1 at approximately 85 kDa. The expected band size for STIM1 is at 77 kDa.
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