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STK16 Antibody

LS-C830304
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSTK16
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    STK16 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:2000 - 1:5000), IHC (1:25 - 1:100), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8576
  • Target name
    STK16
  • Target description
    serine/threonine kinase 16
  • Target synonyms
    KRCT, MPSK, PKL12, PSK, TSF1, hPSK, serine/threonine-protein kinase 16, TGF-beta-stimulated factor 1, myristoylated and palmitoylated serine/threonine-protein kinase, protein kinase PKL12, protein kinase expressed in day 12 fetal liver, tyrosine-protein kinase STK16
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STK16 using anti-STK16 antibody (A10583-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STK16 antigen affinity purified polyclonal antibody (Catalog # A10583-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STK16 at approximately 37 kDa. The expected band size for STK16 is at 35 kDa.
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