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Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human placenta tissue, Primary antibody: CSB-PA083562(STK26 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human placenta tissue, Primary antibody: CSB-PA083562(STK26 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human placenta tissue, Primary antibody: CSB-PA083562(STK26 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes

STK26 Antibody

CSB-PA083562
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetSTK26
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Overview

  • Supplier
    Cusabio
  • Product Name
    STK26 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51765
  • Target name
    STK26
  • Target description
    serine/threonine kinase 26
  • Target synonyms
    MASK, MST4, serine/threonine-protein kinase 26, Mst3 and SOK1-related kinase, STE20-like kinase 4, STE20-like kinase MST4, mammalian Ste20-like protein kinase 4, mammalian sterile 20-like 4, serine/threonine protein kinase 26, serine/threonine protein kinase MST4, serine/threonine-protein kinase MASK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P289
  • Protein Name
    Serine/threonine-protein kinase 26
  • Scientific Description
    The product of this gene is a member of the GCK group III family of kinases, which are a subset of the Ste20-like kinases. The encoded protein contains an amino-terminal kinase domain, and a carboxy-terminal regulatory domain that mediates homodimerization. The protein kinase localizes to the Golgi apparatus and is specifically activated by binding to the Golgi matrix protein GM130. It is also cleaved by caspase-3 in vitro, and may function in the apoptotic pathway. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of MST4/STK26 using anti-MST4/STK26 antibody (A09552-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MST4/STK26 antigen affinity purified polyclonal antibody (Catalog # A09552-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MST4/STK26 at approximately 52 kDa. The expected band size for MST4/STK26 is at 47 kDa.
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