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STK3 antibody detects STK3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with STK3 antibody (GTX104525) diluted by 1:1000.
STK3 antibody detects STK3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with STK3 antibody (GTX104525) diluted by 1:1000.
STK3 antibody detects STK3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with STK3 antibody (GTX104525) diluted by 1:1000.

STK3 antibody

GTX104525
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetSTK3
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Overview

  • Supplier
    GeneTex
  • Product Name
    STK3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.8 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6788
  • Target name
    STK3
  • Target description
    serine/threonine kinase 3
  • Target synonyms
    KRS1, MST2, serine/threonine-protein kinase 3, KB-1458E12.1, MST-2, STE20-like kinase MST2, epididymis secretory sperm binding protein, hippo homolog, mammalian STE20-like protein kinase 2, serine/threonine kinase 3 (STE20 homolog, yeast), serine/threonine kinase 3 (Ste20, yeast homolog), serine/threonine-protein kinase Krs-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13188
  • Protein Name
    Serine/threonine-protein kinase 3
  • Scientific Description
    Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast sterile 20 (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 [PubMed 8816758]; Lee et al., 2001 [PubMed 11278283]).[supplied by OMIM]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STK3/MST-2 using anti-STK3/MST-2 antibody (A02224-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: monkey Cos-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: monkey kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STK3/MST-2 antigen affinity purified polyclonal antibody (Catalog # A02224-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STK3/MST-2 at approximately 56KD. The expected band size for STK3/MST-2 is at 56KD.
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