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ProductsBack/STK3 Mouse Monoclonal Antibody [Clone ID: OTI4G10]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY STK3 (Cat# RC203897, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STK3(Cat# TA500408). Positive lysates [LY401892] (100ug) and [LC401892] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY STK3 (Cat# RC203897, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STK3(Cat# TA500408). Positive lysates [LY401892] (100ug) and [LC401892] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY STK3 (Cat# RC203897, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STK3(Cat# TA500408). Positive lysates [LY401892] (100ug) and [LC401892] (20ug) can be purchased separately from OriGene.

STK3 Mouse Monoclonal Antibody [Clone ID: OTI4G10]

CF500408
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetSTK3
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    STK3 Mouse Monoclonal Antibody [Clone ID: OTI4G10]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4G10
  • Gene ID6788
  • Target name
    STK3
  • Target description
    serine/threonine kinase 3
  • Target synonyms
    KRS1, MST2, serine/threonine-protein kinase 3, KB-1458E12.1, MST-2, STE20-like kinase MST2, epididymis secretory sperm binding protein, hippo homolog, mammalian STE20-like protein kinase 2, serine/threonine kinase 3 (STE20 homolog, yeast), serine/threonine kinase 3 (Ste20, yeast homolog), serine/threonine-protein kinase Krs-1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ13188
  • Protein Name
    Serine/threonine-protein kinase 3
  • Scientific Description
    Carrier-free (BSA/glycerol-free) STK3 mouse monoclonal antibody, clone OTI4G10 (formerly 4G10)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of STK3/MST-2 using anti-STK3/MST-2 antibody (A02224-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: monkey Cos-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: monkey kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STK3/MST-2 antigen affinity purified polyclonal antibody (Catalog # A02224-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STK3/MST-2 at approximately 56KD. The expected band size for STK3/MST-2 is at 56KD.
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