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ProductsBack/STK39 Mouse Monoclonal Antibody [Clone ID: OTI2E10]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY STK39 (Cat# RC223981, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STK39(Cat# TA500497). Positive lysates [LY402227] (100ug) and [LC402227] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY STK39 (Cat# RC223981, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STK39(Cat# TA500497). Positive lysates [LY402227] (100ug) and [LC402227] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY STK39 (Cat# RC223981, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STK39(Cat# TA500497). Positive lysates [LY402227] (100ug) and [LC402227] (20ug) can be purchased separately from OriGene.

STK39 Mouse Monoclonal Antibody [Clone ID: OTI2E10]

CF500497
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSTK39
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    STK39 Mouse Monoclonal Antibody [Clone ID: OTI2E10]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2E10
  • Gene ID27347
  • Target name
    STK39
  • Target description
    serine/threonine kinase 39
  • Target synonyms
    DCHT, PASK, SPAK, STE20/SPS1-related proline-alanine-rich protein kinase, STE20/SPS1 homolog, Ste20-like protein kinase, proline-alanine-rich STE20-related kinase, serine threonine kinase 39 (STE20/SPS1 homolog, yeast), serine/threonine-protein kinase 39, small intestine SPAK-like kinase, ste-20-related kinase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9UEW8
  • Protein Name
    STE20/SPS1-related proline-alanine-rich protein kinase
  • Scientific Description
    Carrier-free (BSA/glycerol-free) STK39 mouse monoclonal antibody, clone OTI2E10 (formerly 2E10)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. IF analysis of SPAK/STK39 using anti-SPAK/STK39 antibody (A02516-3). SPAK/STK39 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5microg/mL rabbit anti-SPAK/STK39 Antibody (A02516-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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