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WB analysis of 293 cell lysate using GTX34235 STMN3 antibody.
WB analysis of 293 cell lysate using GTX34235 STMN3 antibody.
WB analysis of 293 cell lysate using GTX34235 STMN3 antibody.

STMN3 antibody

GTX34235
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetSTMN3
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Overview

  • Supplier
    GeneTex
  • Product Name
    STMN3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID50861
  • Target name
    STMN3
  • Target description
    stathmin 3
  • Target synonyms
    SCG10-like protein; SCLIP; stathmin-3; stathmin-like 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZ72
  • Protein Name
    Stathmin-3
  • Scientific Description
    This gene encodes a protein which is a member of the stathmin protein family. Members of this protein family form a complex with tubulins at a ratio of 2 tubulins for each stathmin protein. Microtubules require the ordered assembly of alpha- and beta-tubulins, and formation of a complex with stathmin disrupts microtubule formation and function. A pseudogene of this gene is located on chromosome 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STMN3 using anti-STMN3 antibody (A08661-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STMN3 antigen affinity purified polyclonal antibody (Catalog # A08661-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STMN3 at approximately 21-25 kDa. The expected band size for STMN3 is at 21 kDa.
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