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STRN3 Antibody

LS-C747681
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetSTRN3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    STRN3 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 77kDa/87kDa, while the observed MW by Western blot was 110kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:100), WB (1:500 - 1:2000) The predicted MW is 77kDa/87kDa, while the observed MW by Western blot was 110kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID29966
  • Target name
    STRN3
  • Target description
    striatin 3
  • Target synonyms
    PPP2R6B, S/G2NA, SG2NA, striatin-3, cell cycle S/G2 nuclear autoantigen, cell cycle autoantigen SG2NA, nuclear autoantigen, protein phosphatase 2 regulatory subunit B'''beta, s/G2 antigen, striatin, calmodulin binding protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STRN3 using anti-STRN3 antibody (A06672-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat H9C2(2-1) whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STRN3 antigen affinity purified polyclonal antibody (Catalog # A06672-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STRN3 at approximately 94 kDa. The expected band size for STRN3 is at 87,78 kDa.
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