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Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Mouse brain tissue, hepg2, 293T and 231 cell, Primary antibody: CSB-PA144649(STX12 Antibody) at dilution 1/800 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Mouse brain tissue, hepg2, 293T and 231 cell, Primary antibody: CSB-PA144649(STX12 Antibody) at dilution 1/800 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Mouse brain tissue, hepg2, 293T and 231 cell, Primary antibody: CSB-PA144649(STX12 Antibody) at dilution 1/800 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds

STX12 Antibody

CSB-PA144649
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSTX12
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Overview

  • Supplier
    Cusabio
  • Product Name
    STX12 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23673
  • Target name
    STX12
  • Target description
    syntaxin 12
  • Target synonyms
    STX13, STX14, syntaxin-12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86Y82
  • Protein Name
    Syntaxin-12
  • Scientific Description
    Syntaxin 12, also known as STX12, STX13 or STX14, is a 276 amino acid single-pass membrane protein that contains one t-SNARE coiled-coil homology domain and belongs to the Syntaxin family. Syntaxin 12 regulates protein transport between late endosomes and the trans-Golgi network and interacts with ABC1 (ATP-binding cassette transporter A1), a protein that facilitates cellular release of choline-phospholipids and cholesterol to apoA-I (apolipoprotein A-I).
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Syntaxin 12/STX12 using anti-Syntaxin 12/STX12 antibody (A09232-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syntaxin 12/STX12 antigen affinity purified polyclonal antibody (Catalog # A09232-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syntaxin 12/STX12 at approximately 37 kDa. The expected band size for Syntaxin 12/STX12 is at 32 kDa.
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