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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA041704(STX18 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA041704(STX18 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA041704(STX18 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)

STX18 Antibody

CSB-PA041704
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSTX18
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Overview

  • Supplier
    Cusabio
  • Product Name
    STX18 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID53407
  • Target name
    STX18
  • Target description
    syntaxin 18
  • Target synonyms
    cell growth-inhibiting gene 9 protein; syntaxin-18; Ufe1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P2W9
  • Protein Name
    Syntaxin-18
  • Scientific Description
    Syntaxins, a family of proteins involved in the fusion of synaptic vesicles with the plasma membrane, display broad tissue distribution and contain carboxy-terminal hydrophobic domains that direct themselves to their respective intracellular compartments. Syntaxin 18 mainly localizes to the endoplasmic reticulum (ER), and functions in transport between the ER and Golgi. Syntaxin 18 also plays a role in ER-mediated phagocytosis, possibly by regulating the specific fusion of the ER and plasma or phagosomal membranes.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Syntaxin 18/STX18 using anti-Syntaxin 18/STX18 antibody (A11895). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syntaxin 18/STX18 antigen affinity purified polyclonal antibody (Catalog # A11895) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syntaxin 18/STX18 at approximately 39 kDa. The expected band size for Syntaxin 18/STX18 is at 39 kDa.
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TargetSTX18
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