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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA126337(STXBP4 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA126337(STXBP4 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA126337(STXBP4 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: x200)

STXBP4 Antibody

CSB-PA126337
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSTXBP4
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Overview

  • Supplier
    Cusabio
  • Product Name
    STXBP4 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID252983
  • Target name
    STXBP4
  • Target description
    syntaxin binding protein 4
  • Target synonyms
    Synip, syntaxin-binding protein 4, STX4-interacting protein, syntaxin 4 interacting protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6ZWJ1
  • Protein Name
    Syntaxin-binding protein 4
  • Scientific Description
    Plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane. Inhibits the translocation of SLC2A4 from intracellular vesicles to the plasma membrane by STX4A binding and preventing the interaction between STX4A and VAMP2. Stimulation with insulin disrupts the interaction with STX4A, leading to increased levels of SLC2A4 at the plasma membrane. May also play a role in the regulation of insulin release by pancreatic beta cells after stimulation by glucose .
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Synip/STXBP4 using anti-Synip/STXBP4 antibody (A08343-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synip/STXBP4 antigen affinity purified polyclonal antibody (Catalog # A08343-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Synip/STXBP4 at approximately 68 kDa. The expected band size for Synip/STXBP4 is at 62 kDa.
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