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SUCLG1 antibody detects SUCLG1 protein by western blot analysis. A.50 μg rat brain lysate/extract 10% SDS-PAGE SUCLG1 antibody (GTX109215) dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SUCLG1 antibody detects SUCLG1 protein by western blot analysis. A.50 μg rat brain lysate/extract 10% SDS-PAGE SUCLG1 antibody (GTX109215) dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SUCLG1 antibody detects SUCLG1 protein by western blot analysis. A.50 μg rat brain lysate/extract 10% SDS-PAGE SUCLG1 antibody (GTX109215) dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SUCLG1 antibody

GTX109215
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSUCLG1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SUCLG1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.48 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8802
  • Target name
    SUCLG1
  • Target description
    succinate-CoA ligase GDP/ADP-forming subunit alpha
  • Target synonyms
    GALPHA, MTDPS9, SUCLA1, succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial, SCS-alpha, mitochondrial succinate--CoA ligase [ADP/GDP-forming] subunit alpha, succinate-CoA ligase alpha subunit, succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial, succinyl-CoA ligase [GDP-forming] subunit alpha, mitochondrial, succinyl-CoA synthetase subunit alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53597
  • Protein Name
    Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial
  • Scientific Description
    This gene encodes the alpha subunit of the heterodimeric enzyme succinate coenzyme A ligase. This enzyme is targeted to the mitochondria and catalyzes the conversion of succinyl CoA and ADP or GDP to succinate and ATP or GTP. Mutations in this gene are the cause of the metabolic disorder fatal infantile lactic acidosis and mitochondrial DNA depletion. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Sundararaman B, Zhan L, Blue SM, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016,61(6):903-13. doi: 10.1016/j.molcel.2016.02.012
    Read this paper
  • Donti TR, Stromberger C, Ge M, et al. Screen for abnormal mitochondrial phenotypes in mouse embryonic stem cells identifies a model for succinyl-CoA ligase deficiency and mtDNA depletion. Dis Model Mech. 2014,7(2):271-80. doi: 10.1242/dmm.013466
    Read this paper

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Figure 1. Western blot analysis of SUCLG1 using anti-SUCLG1 antibody (A06274-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUCLG1 antigen affinity purified polyclonal antibody (Catalog # A06274-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUCLG1 at approximately 35 kDa. The expected band size for SUCLG1 is at 36 kDa.
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