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ProductsBack/SULT1C4 antibody, Internal
WB analysis of MCF-7 cells using GTX46200 SULT1C4 antibody at 0.2-1microg/ml.
WB analysis of MCF-7 cells using GTX46200 SULT1C4 antibody at 0.2-1microg/ml.
WB analysis of MCF-7 cells using GTX46200 SULT1C4 antibody at 0.2-1microg/ml.

SULT1C4 antibody, Internal

GTX46200
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetSULT1C4
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Overview

  • Supplier
    GeneTex
  • Product Name
    SULT1C4 antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID27233
  • Target name
    SULT1C4
  • Target description
    sulfotransferase family 1C member 4
  • Target synonyms
    ST1C4; sulfotransferase 1C2; sulfotransferase 1C4; sulfotransferase family, cytosolic, 1C, member 2; sulfotransferase family, cytosolic, 1C, member 4; sulfotransferase family, cytosolic, 1C, member C2; SULT1C; SULT1C#2; SULT1C2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75897
  • Protein Name
    Sulfotransferase 1C4
  • Scientific Description
    Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a protein that belongs to the SULT1 subfamily, responsible for transferring a sulfo moiety from PAPS to phenol-containing compounds. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SULT1C4 using anti-SULT1C4 antibody (A10623-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SULT1C4 antigen affinity purified polyclonal antibody (Catalog # A10623-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SULT1C4 at approximately 38 kDa. The expected band size for SULT1C4 is at 38 kDa.
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