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ProductsBack/SULT2A1 Mouse Monoclonal Antibody [Clone ID: OTI4D7]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SULT2A1 (Cat# RC204737, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SULT2A1(Cat# TA501694). Positive lysates [LY418856] (100ug) and [LC418856] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SULT2A1 (Cat# RC204737, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SULT2A1(Cat# TA501694). Positive lysates [LY418856] (100ug) and [LC418856] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SULT2A1 (Cat# RC204737, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SULT2A1(Cat# TA501694). Positive lysates [LY418856] (100ug) and [LC418856] (20ug) can be purchased separately from OriGene.

SULT2A1 Mouse Monoclonal Antibody [Clone ID: OTI4D7]

CF501694
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman
TargetSULT2A1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    SULT2A1 Mouse Monoclonal Antibody [Clone ID: OTI4D7]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4D7
  • Gene ID6822
  • Target name
    SULT2A1
  • Target description
    sulfotransferase family 2A member 1
  • Target synonyms
    DHEA-ST, DHEA-ST8, DHEAS, HST, ST2, ST2A1, ST2A3, STD, SULT2A3, hSTa, sulfotransferase 2A1, alcohol/hydroxysteroid sulfotransferase, bile-salt sulfotranasferase 2A1, sulfotransferase family, cytosolic, 2A, dehydroepiandrosterone (DHEA)-preferring, member 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ06520
  • Protein Name
    Sulfotransferase 2A1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) SULT2A1 mouse monoclonal antibody, clone OTI4D7 (formerly 4D7)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of SULT2A1 using anti-SULT2A1 antibody (PB9821). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SULT2A1 antigen affinity purified polyclonal antibody (Catalog # PB9821) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SULT2A1 at approximately 30-35 kDa. The expected band size for SULT2A1 is at 34 kDa.
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