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Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, U87 whole cell lysate, Hela whole cell lysate All lanes: SUMF2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34, 25, 38, 24, 35 kDa Observed band size: 34 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, U87 whole cell lysate, Hela whole cell lysate All lanes: SUMF2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34, 25, 38, 24, 35 kDa Observed band size: 34 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, U87 whole cell lysate, Hela whole cell lysate All lanes: SUMF2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34, 25, 38, 24, 35 kDa Observed band size: 34 kDa

SUMF2 Antibody

CSB-PA839844LA01HU
Cusabio
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetSUMF2
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Overview

  • Supplier
    Cusabio
  • Product Name
    SUMF2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25870
  • Target name
    SUMF2
  • Target description
    sulfatase modifying factor 2
  • Target synonyms
    pFGE, inactive C-alpha-formylglycine-generating enzyme 2, C-alpha-formyglycine-generating enzyme 2, C-alpha-formylglycine-generating enzyme 2, epididymis secretory sperm binding protein, paralog of formylglycine-generating enzyme, paralog of the formylglycine-generating enzyme
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NBJ7
  • Protein Name
    Inactive C-alpha-formylglycine-generating enzyme 2
  • Scientific Description
    Lacks formyl-glycine generating activity and is unable to convert newly synthesized inactive sulfatases to their active form. Inhibits the activation of sulfatases by SUMF1.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SUMF2 using anti-SUMF2 antibody (A08890). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUMF2 antigen affinity purified polyclonal antibody (Catalog # A08890) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUMF2 at approximately 36 kDa. The expected band size for SUMF2 is at 34 kDa.
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