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ICC/IF analysis of C33A cells using GTX00699 SUMO2 + SUMO3 antibody [3H12].
ICC/IF analysis of C33A cells using GTX00699 SUMO2 + SUMO3 antibody [3H12].
ICC/IF analysis of C33A cells using GTX00699 SUMO2 + SUMO3 antibody [3H12].

SUMO2 + SUMO3 antibody [3H12]

GTX00699
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetSUMO3
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Overview

  • Supplier
    GeneTex
  • Product Name
    SUMO2 + SUMO3 antibody [3H12]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. ICC/IF: 1:100-1:500. IHC-Fr: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3H12
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6612
  • Target name
    SUMO3
  • Target description
    small ubiquitin like modifier 3
  • Target synonyms
    SMT3A, SMT3H1, SUMO-3, Smt3B, small ubiquitin-related modifier 3, SMT3 suppressor of mif two 3 homolog 1, SMT3 suppressor of mif two 3 homolog 3, ubiquitin-like protein SMT3B
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDP55854
  • Protein Name
    Small ubiquitin-related modifier 3
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Uchimura Y, Ichimura T, Uwada J, et al. Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation. J Biol Chem. 2006,281(32):23180-90.
    Read this paper

Datasheet

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Figure 1. Western blot analysis of SUMO2/3 using anti-SUMO2/3 antibody (A01282-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 8: mouse SP2/0 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-A01282-2 antigen affinity purified polyclonal antibody (Catalog # A01282-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUMO2/3 at approximately 20 kDa. The expected band size for SUMO2/3 is at 20 kDa.
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