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Western blot All lanes: SUOX antibody at 2microg/ml + Rat liver tissue Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 61 kDa Observed band size: 61 kDa
Western blot All lanes: SUOX antibody at 2microg/ml + Rat liver tissue Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 61 kDa Observed band size: 61 kDa
Western blot All lanes: SUOX antibody at 2microg/ml + Rat liver tissue Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 61 kDa Observed band size: 61 kDa

SUOX Antibody

CSB-PA022954HA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetSUOX
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Overview

  • Supplier
    Cusabio
  • Product Name
    SUOX Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6821
  • Target name
    SUOX
  • Target description
    sulfite oxidase
  • Target synonyms
    sulfite oxidase, mitochondrial
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51687
  • Protein Name
    Sulfite oxidase, mitochondrial
  • Scientific Description
    Sulfite oxidase is a homodimeric protein localized to the intermembrane space of mitochondria. Each subunit contains a heme domain and a molybdopterin-binding domain. The enzyme catalyzes the oxidation of sulfite to sulfate, the final reaction in the oxidative degradation of the sulfur amino acids cysteine and methionine. Sulfite oxidase deficiency results in neurological abnormalities which are often fatal at an early age. Alternative splicing results in multiple transcript variants encoding identical proteins.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Sulfite oxidase/SUOX using anti-Sulfite oxidase/SUOX antibody (A05838). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sulfite oxidase/SUOX antigen affinity purified polyclonal antibody (Catalog # A05838) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sulfite oxidase/SUOX at approximately 60 kDa. The expected band size for Sulfite oxidase/SUOX is at 60 kDa.
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