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ProductsBack/SURF6 Rabbit Polyclonal Antibody
Gel: 8%SDS-PAGE Lysate: 40 microg Lane 1-3: Hela K562 and HL-60 cell lysates Primary antibody: TA365047 (SURF6 Antibody) at dilution 1/700 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 10 seconds
Gel: 8%SDS-PAGE Lysate: 40 microg Lane 1-3: Hela K562 and HL-60 cell lysates Primary antibody: TA365047 (SURF6 Antibody) at dilution 1/700 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 10 seconds
Gel: 8%SDS-PAGE Lysate: 40 microg Lane 1-3: Hela K562 and HL-60 cell lysates Primary antibody: TA365047 (SURF6 Antibody) at dilution 1/700 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 10 seconds

SURF6 Rabbit Polyclonal Antibody

TA365047
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
TargetSURF6
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    SURF6 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID6838
  • Target name
    SURF6
  • Target description
    surfeit 6
  • Target synonyms
    RRP14; surfeit locus protein 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75683
  • Protein Name
    Surfeit locus protein 6
  • Scientific Description
    SURF6 rabbit polyclonal antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SURF6 using anti-SURF6 antibody (A11571-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SURF6 antigen affinity purified polyclonal antibody (Catalog # A11571-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SURF6 at approximately 45 kDa. The expected band size for SURF6 is at 41 kDa.
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