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WB analysis of human lymph node lysate using GTX89933 SWAP70 antibody, C-term. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human lymph node lysate using GTX89933 SWAP70 antibody, C-term. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human lymph node lysate using GTX89933 SWAP70 antibody, C-term. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer

SWAP70 antibody, C-term

GTX89933
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetSWAP70
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Overview

  • Supplier
    GeneTex
  • Product Name
    SWAP70 antibody, C-term
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 0.1-0.3microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23075
  • Target name
    SWAP70
  • Target description
    switching B cell complex subunit SWAP70
  • Target synonyms
    HSPC321; SWAP switching B-cell complex 70kDa subunit; SWAP switching B-cell complex subunit 70; SWAP-70; switch-associated protein 70
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ9UH65
  • Protein Name
    Switch-associated protein 70
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SWAP70 using anti-SWAP70 antibody (A06972-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SWAP70 antigen affinity purified polyclonal antibody (Catalog # A06972-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SWAP70 at approximately 69 kDa. The expected band size for SWAP70 is at 69 kDa.
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