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The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using CSB-PA187364(SWAP70 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using CSB-PA187364(SWAP70 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using CSB-PA187364(SWAP70 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)

SWAP70 Antibody

CSB-PA187364
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSWAP70
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Overview

  • Supplier
    Cusabio
  • Product Name
    SWAP70 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23075
  • Target name
    SWAP70
  • Target description
    switching B cell complex subunit SWAP70
  • Target synonyms
    HSPC321; SWAP switching B-cell complex 70kDa subunit; SWAP switching B-cell complex subunit 70; SWAP-70; switch-associated protein 70
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UH65
  • Protein Name
    Switch-associated protein 70
  • Scientific Description
    Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion (By similarity).
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SWAP70 using anti-SWAP70 antibody (A06972-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SWAP70 antigen affinity purified polyclonal antibody (Catalog # A06972-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SWAP70 at approximately 69 kDa. The expected band size for SWAP70 is at 69 kDa.
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