Swine anti Horse Ig light chains kappa and lambda (free and bound)

Nordic-MUbio

Swine anti Horse Ig light chains kappa and lambda (free and bound)

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Precipitating polyclonal Swine antiSerum to Horse light chains, free and bound.

Precipitation assays. In immunoelectrophoresis use 2 microl or equivalent against 120 microl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 microl antiserum in a 3 mm diameter centre well and 2 microl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single immunodiffusion, use 1% antiserum in agar gel to establish a standard curve.

Secondary antibodies

Research Use Only

Polyclonal

Porcine

Purified equine light chains isolated from polyclonal horse IgG. Freunds complete adjuvant is used in the first step of the immunization procedure.

12352203