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WB analysis of various sample lysates using GTX53913 SynCAM antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX53913 SynCAM antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX53913 SynCAM antibody. Dilution : 1:1000 Loading : 25microg per lane

SynCAM antibody

GTX53913
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetCADM1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SynCAM antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23705
  • Target name
    CADM1
  • Target description
    cell adhesion molecule 1
  • Target synonyms
    BL2, IGSF4, IGSF4A, NECL2, Necl-2, RA175, ST17, SYNCAM, TSLC1, sTSLC-1, sgIGSF, synCAM1, cell adhesion molecule 1, TSLC-1, TSLC1/Nectin-like 2/IGSF4, immunoglobulin superfamily member 4, nectin-like 2, nectin-like protein 2, spermatogenic immunoglobulin superfamily, synaptic cell adhesion molecule, tumor suppressor in lung cancer 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BY67
  • Protein Name
    Cell adhesion molecule 1
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 2. Flow Cytometry analysis of A549 cells using anti-SynCAM antibody (PB9136). Overlay histogram showing A549 cells stained with PB9136 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SynCAM Antibody (PB9136, 1 microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 microg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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