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ProductsBack/SYNE2 / Nesprin-2 Antibody (aa1075-1125)

SYNE2 / Nesprin-2 Antibody (aa1075-1125)

LS-C762907
LifeSpan BioSciences
ApplicationsImmunoPrecipitation
Product group Antibodies
TargetSYNE2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SYNE2 / Nesprin-2 Antibody (aa1075-1125)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunoprecipitation Range: 2-10 microg/mg lysate
  • Applications
    ImmunoPrecipitation
  • Applications Supplier
    IP Immunoprecipitation Range: 2-10 µg/mg lysate WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23224
  • Target name
    SYNE2
  • Target description
    spectrin repeat containing nuclear envelope protein 2
  • Target synonyms
    EDMD5, KASH2, NUA, NUANCE, Nesp2, Nesprin-2, SYNE-2, TROPH, nesprin-2, KASH domain-containing protein 2, nesprin 2, nesprin-2 spectrin repeats 3-54, nuclear envelope spectrin repeat protein 2, nucleus and actin connecting element protein, polytrophin, spectrin repeat containing, nuclear envelope 2, synaptic nuclear envelope protein 2, synaptic nuclei expressed gene 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Nesprin 2/SYNE2 using anti-Nesprin 2/SYNE2 antibody (A02818-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nesprin 2/SYNE2 antigen affinity purified polyclonal antibody (Catalog # A02818-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nesprin 2/SYNE2 at approximately 50-60 kDa. The expected band size for Nesprin 2/SYNE2 is at 796 kDa.
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