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Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA023019LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA023019LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA023019LA01HU at dilution of 1:100

SYNM Antibody

CSB-PA023019LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSYNM
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Overview

  • Supplier
    Cusabio
  • Product Name
    SYNM Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23336
  • Target name
    SYNM
  • Target description
    synemin
  • Target synonyms
    DMN, SYN, synemin, desmuslin, synemin alpha, synemin beta, synemin, intermediate filament protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15061
  • Protein Name
    Synemin
  • Scientific Description
    Type-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteropolymeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteropolymeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SYNM using anti-SYNM antibody (A06653-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYNM antigen affinity purified polyclonal antibody (Catalog # A06653-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYNM at approximately 180 kDa. The expected band size for SYNM is at 173 kDa.
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Immunohistochemistry analysis in human skeletal muscle and liver tissues using HPA040066 antibody. Corresponding SYNM RNA-seq data are presented for the same tissues.
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