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ProductsBack/Syntaxin Binding Protein 1 Antibody (YA2620, Rabbit)

Syntaxin Binding Protein 1 Antibody (YA2620, Rabbit)

HY-P82875
MedChem Express
TargetSTXBP1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Syntaxin Binding Protein 1 Antibody (YA2620, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID6812
  • Target name
    STXBP1
  • Target description
    syntaxin binding protein 1
  • Target synonyms
    DEE4, MUNC18-1, N-Sec1, NSEC1, P67, RBSEC1, UNC18, unc-18A, unc18-1, syntaxin-binding protein 1, neuronal SEC1, protein unc-18 homolog 1, protein unc-18 homolog A
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Syntaxin Binding Protein 1 Antibody (YA2620) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2620), targeting Syntaxin Binding Protein 1, with a predicted molecular weight of 68 kDa (observed band size: 65 kDa). Syntaxin Binding Protein 1 Antibody (YA2620) can be used for WB, IHC-P, ICC/IF experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of STXBP1 using anti-STXBP1 antibody (PB9819). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STXBP1 antigen affinity purified polyclonal antibody (Catalog # PB9819) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STXBP1 at approximately 70 kDa. The expected band size for STXBP1 is at 67 kDa.
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