T7 RNA Polymerase HC, Highly concentrated T7 RNA Polymerase for set-up of high yield in vitro transcription reactions

Jena Bioscience

T7 RNA Polymerase HC, Highly concentrated T7 RNA Polymerase for set-up of high yield in vitro transcription reactions

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Research Use Only

Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase (99 kDa) that catalyzes in vitro RNA synthesis from a DNA sequence containing a T7 phage promoter. The highly concentrated version T7 RNA Polymerase HC is ideally suited for set-up and optimization of high yield in vitro transcription reactions. The provided T7 IVT Set-up Buffer, MgCl2 and DTT solutions enable testing of different reaction conditions such as nucleotide/ MgCl2 concentrations. Recommendation for assay set-up with minimal optimization: HighYield T7 RNA Synthesis Kit (#RNT-101). Activity: 1 microl T7 RNA Polymerase HC generates >/= 30 microg RNA in 10 min at 37°. This is comparable to 1000 U of NEBs T7 RNA Polymerase (High Concentration), #M0460T (activity assay conditions: 1x T7 IVT Set-up Buffer supplemented with 10 mM DTT, 12 mM MgCl2, 3 mM NTPs, 1 microl T7 RNA Polymerase HC and 1 microg DNA template (1.4 kpb RNA transcript) in 20 microl. RNA yield was determined with a fluorescence microplate assay.)

-20°C

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