Figure 1. Immunofluorescence of Substance P staining in Vibratome section of Rat spinal cord. The section was incubated with at 1/500 for 48h, followed by an appropriate secondary antibody coupled to Cyanine 5. This antibody stains SP-Positive nerve (blue) in the close vicinity of Neurons expressing Neurokinin 1 receptor (Green) and 5-Alpha Reductase (Red) in the dorsal horn laminal. Scal bar - 20microm. Patte-Mensch C et al. (2005) Proc Natl. Acad. Sci. USA 102 (25):9044-9.