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TACC1 Antibody

CSB-PA050197
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTACC1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TACC1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6867
  • Target name
    TACC1
  • Target description
    transforming acidic coiled-coil containing protein 1
  • Target synonyms
    Ga55, transforming acidic coiled-coil-containing protein 1, gastric cancer antigen Ga55, taxin-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75410
  • Protein Name
    Transforming acidic coiled-coil-containing protein 1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TACC1 using anti-TACC1 antibody (A04196). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: mouse testis tissue lysate, lane 2: U87 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TACC1 antigen affinity purified polyclonal antibody (Catalog # A04196) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TACC1 at approximately 100KD. The expected band size for TACC1 is at 88KD.
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