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Western Blot analysis of HepG2 cells using TAF II p68 Polyclonal Antibody
Western Blot analysis of HepG2 cells using TAF II p68 Polyclonal Antibody
Western Blot analysis of HepG2 cells using TAF II p68 Polyclonal Antibody

TAF15 Antibody

CSB-PA004225
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTAF15
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Overview

  • Supplier
    Cusabio
  • Product Name
    TAF15 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8148
  • Target name
    TAF15
  • Target description
    TATA-box binding protein associated factor 15
  • Target synonyms
    Npl3; RBP56; RBP56/CSMF fusion; TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa; TAF2N; TAFII68; TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD (RNA-binding protein 56); TATA box-binding protein-associated factor 2N (RNA-binding protein 56); TATA-binding protein-associated factor 2N; TBP-associated factor 15
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92804
  • Protein Name
    TATA-binding protein-associated factor 2N
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TAF15 using anti-TAF15 antibody (M03567-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat NRK whole cell lysates, Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAF15 antigen affinity purified monoclonal antibody (Catalog # M03567-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAF15 at approximately 75 kDa. The expected band size for TAF15 is at 62 kDa.
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