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ProductsBack/TAK1 (MAP3K7) Rabbit Polyclonal Antibody
Gel: 8%SDS-PAGE Lysate: 40 microg Lane 1-4: K562 Jurkat HepG2 and 231 cell lysates Primary antibody: TA365055 (MAP3K7 Antibody) at dilution 1/1000 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 1 minute
Gel: 8%SDS-PAGE Lysate: 40 microg Lane 1-4: K562 Jurkat HepG2 and 231 cell lysates Primary antibody: TA365055 (MAP3K7 Antibody) at dilution 1/1000 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 1 minute
Gel: 8%SDS-PAGE Lysate: 40 microg Lane 1-4: K562 Jurkat HepG2 and 231 cell lysates Primary antibody: TA365055 (MAP3K7 Antibody) at dilution 1/1000 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 1 minute

TAK1 (MAP3K7) Rabbit Polyclonal Antibody

TA365055
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
TargetMAP3K7
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    TAK1 (MAP3K7) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID6885
  • Target name
    MAP3K7
  • Target description
    mitogen-activated protein kinase kinase kinase 7
  • Target synonyms
    CSCF; FMD2; MEKK7; mitogen-activated protein kinase kinase kinase 7; TAK1; TGF1a; TGF-beta activated kinase 1; transforming growth factor-beta-activated kinase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43318
  • Protein Name
    Mitogen-activated protein kinase kinase kinase 7
  • Scientific Description
    MAP3K7 rabbit polyclonal antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TAK1 using anti-TAK1 antibody (PB10006). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human A374 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAK1 antigen affinity purified polyclonal antibody (Catalog # PB10006) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAK1 at approximately 72 kDa. The expected band size for TAK1 is at 67 kDa.
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